5FL7

Structure of the F1c10 complex from Yarrowia lipolytica ATP synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-05
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 2
Unit cell lengths	169.500, 182.200, 193.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.366 - 3.500
R-factor	0.2747
Rwork	0.274
R-free	0.30540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xok
RMSD bond length	0.004
RMSD bond angle	0.448
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.190	3.600
High resolution limit [Å]	3.500	3.500
Rmerge	0.180
Number of reflections	75882
<I/σ(I)>	9.84	0.61
Completeness [%]	99.9	100
Redundancy	19.47	19.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		100 MM TRIS-HCL, POLYETHYLENE GLYCOL 400, pH 8.0