5FKP
Crystal structure of the mouse CD1d in complex with the p99 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.170, 107.570, 110.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.190 - 1.800 |
| R-factor | 0.20681 |
| Rwork | 0.205 |
| R-free | 0.23361 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g08 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.267 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.830 |
| Number of reflections | 47272 | |
| <I/σ(I)> | 11.1 | 2.4 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 6.1 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2M AMMONIUM TARTRATE AND 20% PEG4000 |
