5FJV
Crystal structure of the extracellular domain of alpha2 nicotinic acetylcholine receptor in pentameric assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-30 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.524, 116.559, 129.546 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.263 - 3.200 |
| R-factor | 0.2279 |
| Rwork | 0.224 |
| R-free | 0.30790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uv6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.977 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.260 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.090 | 0.880 |
| Number of reflections | 26037 | |
| <I/σ(I)> | 18.2 | 2.6 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6.6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 100 MM BICINE PH 9.5, 20% PEG 8000 |
