5FJS
Bacterial beta-glucosidase reveals the structural and functional basis of genetic defects in human glucocerebrosidase 2 (GBA2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 120 |
| Detector technology | PIXEL |
| Collection date | 2014-04-05 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 61 |
| Unit cell lengths | 187.974, 187.974, 99.075 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 162.610 - 2.600 |
| R-factor | 0.20862 |
| Rwork | 0.206 |
| R-free | 0.26649 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5bvu |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.561 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.100 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.110 | 1.320 |
| Number of reflections | 61406 | |
| <I/σ(I)> | 15.9 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.3 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M AMMONIUM SULFATE, 0.1 M BIS-TRIS PROPANE, PH 8, 15 % W/V PEG 6000. |
