5FG3
Crystal structure of GDP-bound aIF5B from Aeropyrum pernix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-11-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.853, 97.448, 61.296 |
| Unit cell angles | 90.00, 94.77, 90.00 |
Refinement procedure
| Resolution | 37.210 - 1.900 |
| R-factor | 0.19668 |
| Rwork | 0.194 |
| R-free | 0.24293 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g7s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.446 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.210 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.065 | 0.305 |
| Number of reflections | 50764 | |
| <I/σ(I)> | 12.3 | 3.7 |
| Completeness [%] | 95.4 | 88.1 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Tris-HCl (pH7.5), 20%(w/v) PEG 3000, 0.2 M calcium acetate |
