5FCR

MOUSE COMPLEMENT FACTOR D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-08-16
Detector	PSI PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	P 1
Unit cell lengths	51.271, 52.866, 84.278
Unit cell angles	90.46, 98.04, 90.14

Refinement procedure

Resolution	83.450 - 1.250
R-factor	0.15209
Rwork	0.150
R-free	0.19599
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5fck
RMSD bond length	0.032
RMSD bond angle	2.700
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.350	1.280
High resolution limit [Å]	1.250	1.250
Rmerge	0.040	0.720
Number of reflections	215515
<I/σ(I)>	13.9
Completeness [%]	88.3	75.1
Redundancy	1.8	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Mouse FD was crystallized by the sitting drop vapour diffusion method. A 1 uL FD solution (14.4 mg/mL FD, 50 mM Tris pH 8.0, 100 mM NaCl) was mixed with 1 uL reservoir solution (0.1 M HEPES pH 7.0, 30% v/v Jeffamine ED-2001 pH 7.0) and equilibrated against 200 uL reservoir solution.