5FCN
microtubule binding domain of human CEP135
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-19 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 78.850, 78.850, 95.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.420 - 1.800 |
| R-factor | 0.2167 |
| Rwork | 0.216 |
| R-free | 0.23790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gkw |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.373 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1965) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.420 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.042 | 0.421 |
| Number of reflections | 14195 | |
| <I/σ(I)> | 18.71 | 3.49 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 4.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.15 | 25% PEG 1500, 0.1M SPG buffer |
