5FBW
PI4KB in complex with Rab11 and the MI369 Inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.066, 104.091, 187.559 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.469 - 3.487 |
| R-factor | 0.248 |
| Rwork | 0.246 |
| R-free | 0.28310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d0l |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.147 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.470 | 3.610 |
| High resolution limit [Å] | 3.480 | 3.480 |
| Rmerge | 0.191 | |
| Total number of observations | 54212 | |
| Number of reflections | 12696 | |
| <I/σ(I)> | 6.77 | 0.87 |
| Completeness [%] | 98.3 | |
| Redundancy | 4.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M ammonium sulfate, 0.1M MES pH=6.5, 20% (w/v) PEG 8000 |
