5FAP
CTX-M-15 in complex with FPI-1602
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-15 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.120, 61.830, 73.070 |
| Unit cell angles | 90.00, 103.98, 90.00 |
Refinement procedure
| Resolution | 30.970 - 2.700 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hbt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.256 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.970 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 14478 |
| <I/σ(I)> | 11.7 |
| Completeness [%] | 95.4 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2M ammonium sulfate, 0.1M MES pH6.5, 30% PEG5K monomethyl ether |
