5FAF
N184K pathological variant of gelsolin domain 2 (orthorhombic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 26.390, 50.120, 80.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.500 - 1.050 |
| R-factor | 0.13204 |
| Rwork | 0.130 |
| R-free | 0.16816 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kcq |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.167 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.500 | 1.110 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.050 | |
| Number of reflections | 48782 | |
| <I/σ(I)> | 8.3 | 2.4 |
| Completeness [%] | 96.8 | 91.4 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 20% PEG 8000 0.1 M MES 0.2 M calcium acetate |
