5F9G
pnGFP1.5-Y.Cro: circularly permuted green fluorescent protein (with a tyrosine-derived chromophore)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.987 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 57.258, 57.258, 185.392 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.682 - 2.772 |
R-factor | 0.2075 |
Rwork | 0.203 |
R-free | 0.29470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1emb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.442 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.089 | 0.920 |
Number of reflections | 14821 | |
<I/σ(I)> | 80.4 | 8 |
Completeness [%] | 99.8 | 100 |
Redundancy | 24.7 | 27.1 |
CC(1/2) | 0.968 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | pnGFP1.5-Y.Cro in buffer 50mM Hepes pH 7.5, 0.3M NaCl, 1mM beta-mercaptoethanol, A280=52; Crystals were grown in drops of 1ul protein solution: 1ul well solution containing 20-22% PEG 3350, 0.25M potassium thiocyanate, 0.1M Tris pH 8.0 |