5F8P

A Novel Inhibitor of the Obesity-Related Protein FTO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-01
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	H 3
Unit cell lengths	142.218, 142.218, 83.028
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.870 - 2.200
R-factor	0.1901
Rwork	0.188
R-free	0.23390
RMSD bond length	0.011
RMSD bond angle	1.303
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	71.109
High resolution limit [Å]	2.199
Number of reflections	31828
<I/σ(I)>	30.1
Completeness [%]	96.7
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10 %(w/v) polyethylene glycol (PEG) 3350, 0.1 M trisodium citrate dehydrate, 3.0 mM N-oxalylglycine (NOG)