5F8H

Enterovirus 71 Polymerase Elongation Complex (C1S1/2 Form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97892
Spacegroup name	P 21 21 21
Unit cell lengths	63.344, 77.081, 149.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.957 - 2.450
R-factor	0.2004
Rwork	0.198
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ol6
RMSD bond length	0.008
RMSD bond angle	1.130
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (phenix.refine: 1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	60.000	2.540
High resolution limit [Å]	2.450	5.280	2.450
Rmerge	0.062	0.028	0.509
Rmeas	0.070	0.032	0.574
Rpim	0.032	0.015	0.259
Total number of observations	130908
Number of reflections	27772
<I/σ(I)>	11
Completeness [%]	99.7	98.2	99.9
Redundancy	4.7	4.4	4.8
CC(1/2)		0.999	0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	0.085M MES pH 6.5, 25.5%(w/v) PEG 5000 monomethyl ether, 15%(v/v) Glycerol