5F86
Crystal structure of Drosophila Poglut1 (Rumi) complexed with its substrate protein (EGF repeat)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 270.250, 270.250, 47.419 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.19682 |
| Rwork | 0.196 |
| R-free | 0.22120 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.184 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.083 | 0.624 |
| Number of reflections | 51561 | |
| <I/σ(I)> | 24.4 | 1.9 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 8.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.62M ammonium sulfate |
