5F6K
Crystal structure of the MLL3-Ash2L-RbBP5 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-10 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.1272 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 80.342, 236.076, 44.416 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.110 | 0.049 | 0.654 |
Rmeas | 0.118 | 0.054 | 0.703 |
Rpim | 0.044 | 0.021 | 0.256 |
Total number of observations | 239734 | ||
Number of reflections | 33535 | ||
<I/σ(I)> | 11.4 | ||
Completeness [%] | 99.9 | 99.3 | 100 |
Redundancy | 7.1 | 6.5 | 7.3 |
CC(1/2) | 0.998 | 0.939 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100 mM Na-Cacodylate, pH 6.5, 10% PEG 3350, 0.1 M MgCl2 |