5F6C
The structure of E. coli RNase E catalytically inactive mutant with RNA bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-01-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97778 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.619, 122.562, 122.195 |
| Unit cell angles | 90.00, 99.77, 90.00 |
Refinement procedure
| Resolution | 54.616 - 3.002 |
| R-factor | 0.1787 |
| Rwork | 0.175 |
| R-free | 0.24980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.628 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 120.400 |
| High resolution limit [Å] | 3.001 |
| Number of reflections | 26464 |
| <I/σ(I)> | 14.04 |
| Completeness [%] | 99.2 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1 M Tris pH 8.5, 0.2 M MgCl2 and 20% wt/v PEG 8000. |
