5F5O
Crystal structure of Marburg virus nucleoprotein core domain bound to VP35 regulation peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-02 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 32 |
| Unit cell lengths | 98.396, 98.396, 95.730 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 31.910 - 2.200 |
| R-factor | 0.21722 |
| Rwork | 0.214 |
| R-free | 0.27733 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.847 |
| Data reduction software | SCALEPACK |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 52682 |
| <I/σ(I)> | 36.78 |
| Completeness [%] | 100.0 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 200 mM Ammonium sulfate, 100 mM Tris pH 8.5, 18% (wt/vol) Polyethylene glycol 3350 |
