5F5G
Structure of E.Coli GlpG Y205F mutant complexed with peptidic inhibitor Ac-RMA-CHO in the DMPC/CHAPSO bicelle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-17 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.981 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 70.830, 97.500, 62.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.010 - 2.300 |
R-factor | 0.20911 |
Rwork | 0.207 |
R-free | 0.25476 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.485 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 10422 | |
<I/σ(I)> | 6.7 | |
Completeness [%] | 91.3 | |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.5M NaCl, 0.1M sodium acetate pH 4.5, 5% Glycerol, 7% DMPC/CHAPSO bicelle |