5F5C
Crystal Structure of human JMJD2D complexed with KDOPP7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-28 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.594, 71.594, 150.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.920 - 1.880 |
| R-factor | 0.1734 |
| Rwork | 0.172 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d6q |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.693 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.680 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.129 | 0.518 |
| Number of reflections | 32698 | |
| <I/σ(I)> | 13.5 | 1.96 |
| Completeness [%] | 99.8 | 97.5 |
| Redundancy | 10.4 | 3.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 26% PEG3350 , 0.1M HEPES pH 6.8 , 0.2M ammonium sulfate |
