5F28

Crystal structure of FAT domain of Focal Adhesion Kinase (FAK) bound to the transcription factor MEF2C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8729
Spacegroup name	P 42 21 2
Unit cell lengths	139.210, 139.210, 90.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.221 - 2.900
R-factor	0.2097
Rwork	0.208
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1K40 (FAT) and 3KOV (MEF2)
RMSD bond length	0.004
RMSD bond angle	0.593
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_2196))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.220	3.080
High resolution limit [Å]	2.900	2.900
Rmerge	0.344	1.559
Number of reflections	37566
<I/σ(I)>	8.7	1.9
Completeness [%]	100.0	100
Redundancy	13.7	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	0.1M Magnseium acetate, 0.1M MES, pH 6.5, 12% PEG 8000