5F1Q

Crystal Structure of Periplasmic Dipeptide Transport Protein from Yersinia pestis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97916
Spacegroup name	C 1 2 1
Unit cell lengths	289.290, 59.745, 80.631
Unit cell angles	90.00, 103.96, 90.00

Refinement procedure

Resolution	28.311 - 1.956
R-factor	0.1674
Rwork	0.166
R-free	0.20020
RMSD bond length	0.009
RMSD bond angle	1.131
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX ((phenix.refine: dev_1839))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.980
High resolution limit [Å]	1.950	1.950
Rmerge		0.513
Number of reflections	93243
<I/σ(I)>	7.98	1.42
Completeness [%]	98.4	98.3
Redundancy	4.2	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	289	0.1 M phosphate-citrate, pH 4.2, 40:5(v/v) PEG 600