5F17
Structure of EAV NSP11 K170A mutant at 3.19A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 249.184, 249.184, 226.899 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 3.200 |
R-factor | 0.2162 |
Rwork | 0.215 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eyi |
RMSD bond length | 0.004 |
RMSD bond angle | 0.864 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 44774 | |
<I/σ(I)> | 23 | 1.7 |
Completeness [%] | 99.7 | 100 |
Redundancy | 7.6 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG |