5F0L
Structure of retromer VPS26-VPS35 subunits bound to SNX3 and DMT1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97903 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 371.370, 75.210, 57.450 |
| Unit cell angles | 90.00, 98.56, 90.00 |
Refinement procedure
| Resolution | 56.800 - 3.200 |
| R-factor | 0.2141 |
| Rwork | 0.212 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f0j |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.153 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.820 | 3.380 | |
| High resolution limit [Å] | 3.190 | 9.450 | 3.190 |
| Rmerge | 0.183 | 0.040 | 0.929 |
| Rmeas | 0.198 | 0.044 | 1.006 |
| Total number of observations | 178697 | ||
| Number of reflections | 26241 | 1059 | 4074 |
| <I/σ(I)> | 9.3 | 37.64 | 1.82 |
| Completeness [%] | 99.3 | 99.3 | 96.2 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.75 M AmSO4, 0.1 M MES pH 6.0, 15% Glycerol |
