5F0J

Structure of retromer VPS26-VPS35 subunits bound to SNX3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97893
Spacegroup name	C 1 2 1
Unit cell lengths	372.820, 75.580, 57.530
Unit cell angles	90.00, 97.87, 90.00

Refinement procedure

Resolution	56.980 - 2.700
R-factor	0.2091
Rwork	0.207
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fau 2yps
RMSD bond length	0.008
RMSD bond angle	1.198
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	57.000		2.850
High resolution limit [Å]	2.690	7.990	2.690
Rmerge	0.090	0.028	0.800
Rmeas	0.107	0.033	0.951
Total number of observations	292428
Number of reflections	85401	3284	13008
<I/σ(I)>	12.09	38.87	1.51
Completeness [%]	98.3	99	92.6
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.9 M AmSO4, 0.1 M MES pH 6.0