5EZQ
Venezuelan Equine Encephalitis Virus (VEEV) Nonstructural protein 2 (nsP2) Cysteine Protease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2015-01-16 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.805, 63.720, 86.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.210 - 1.660 |
| R-factor | 0.20271 |
| Rwork | 0.202 |
| R-free | 0.21088 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hwk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.163 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.720 | 1.760 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.375 | |
| Number of reflections | 40129 | |
| <I/σ(I)> | 21.91 | 3.24 |
| Completeness [%] | 99.6 | 97.7 |
| Redundancy | 6.01 | 2.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | JCSG+ Condition #15 (0.1 M Bicine pH 8.5, 20% PEG 6000) |
