5EZ5

Crystal structure of active Rab11A (S20V) in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	295
Detector technology	CCD
Collection date	2013-06-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	74.111, 74.111, 149.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.340 - 2.400
R-factor	0.24033
Rwork	0.237
R-free	0.30380
RMSD bond length	0.018
RMSD bond angle	2.005
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	15662
<I/σ(I)>	30.8
Completeness [%]	100.0
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Sodium chloride, Tris, Magnessium chloride