5EY0

Crystal structure of CodY from Staphylococcus aureus with GTP and Ile

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2012-05-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	45.894, 76.028, 158.023
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.941 - 1.600
R-factor	0.1793
Rwork	0.178
R-free	0.20910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2B0L and 2B18
RMSD bond length	0.007
RMSD bond angle	1.076
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.072	0.313
Number of reflections	69602
<I/σ(I)>	23.72	2.8
Completeness [%]	94.3	92.8
Redundancy	3.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.9	293	ammonium acetate, sodium citrate dehydrate, PEG400