5EW4
Crystal structure of C9ORF72 Antisense CCCCGG repeat RNA associated with Lou Gehrig's disease and frontotemporal dementia, crystallized with Sr2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97924 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.020, 47.615, 58.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.923 - 1.470 |
R-factor | 0.2179 |
Rwork | 0.215 |
R-free | 0.23890 |
Structure solution method | AB INITIO |
RMSD bond length | 0.024 |
RMSD bond angle | 0.508 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | ACORN |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.500 |
High resolution limit [Å] | 1.470 | 3.990 | 1.470 |
Rmerge | 0.032 | 0.019 | 0.542 |
Rmeas | 0.036 | 0.022 | 0.648 |
Rpim | 0.016 | 0.010 | 0.343 |
Total number of observations | 90931 | ||
Number of reflections | 20000 | ||
<I/σ(I)> | 14.1 | ||
Completeness [%] | 98.6 | 91.7 | 89.4 |
Redundancy | 4.5 | 4.4 | 2.9 |
CC(1/2) | 0.998 | 0.713 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.04 M sodium cacodylate, 50 mM KCl, 0.04 M LiCl, 0.012 M spermine, 0.08 M strontium chloride, 10% (v/v) (+/-)-2-methyl-2,4-pentanediol, equilibrated against 35% (v/v) (+/-)-2-methyl-2,4-pentanediol |