5EUC
The role of the C-terminal region on the oligomeric state and enzymatic activity of Trypanosoma cruzi hypoxanthine phosphoribosyl transferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-02 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.800000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 95.667, 95.667, 75.685 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.340 - 2.650 |
| R-factor | 0.1933 |
| Rwork | 0.190 |
| R-free | 0.26370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tc2 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.539 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.340 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 22531 | |
| <I/σ(I)> | 13.6 | 3.1 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | The drop was a 1:1 mix of protein (in tris 20 mM pH8, NaCl 100 mM) and buffer (1.0 mM MES buffer pH 6.5 and 12 % W/V of PEG 20000) |
