5ETC

Structure of inactive MAPK14 with ordered Activation Loop

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-22
Detector	MAR CCD 130 mm
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	45.128, 86.880, 124.018
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.472 - 2.422
R-factor	0.1969
Rwork	0.194
R-free	0.24250
RMSD bond length	0.006
RMSD bond angle	0.955
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.472
High resolution limit [Å]	2.420
Number of reflections	16774
<I/σ(I)>	11
Completeness [%]	87.3
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.5 to 1.6 M (NH4)2SO4, 50mM MgCl2 and 50 mM TRIS/HCl pH 8.0