5ESZ
Crystal Structure of Broadly Neutralizing Antibody CH04, Isolated from Donor CH0219, in Complex with Scaffolded Trimeric HIV-1 Env V1V2 Domain from the Clade AE Strain A244
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 63 |
| Unit cell lengths | 116.716, 116.716, 249.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.774 - 4.191 |
| R-factor | 0.2547 |
| Rwork | 0.252 |
| R-free | 0.28210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5esv |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.829 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2243: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.320 |
| High resolution limit [Å] | 4.190 | 4.190 |
| Rmerge | 0.097 | 0.769 |
| Number of reflections | 13064 | |
| <I/σ(I)> | 19.2 | 1.8 |
| Completeness [%] | 95.8 | 77.6 |
| Redundancy | 9.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 54% isopropanol .1M Tris pH 8.5 Cryo used was 15% 2R3R |
