5ERD
Crystal structure of human Desmoglein-2 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.968, 114.476, 226.127 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 84.395 - 2.900 |
R-factor | 0.2437 |
Rwork | 0.241 |
R-free | 0.28680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3q2w |
RMSD bond length | 0.044 |
RMSD bond angle | 1.270 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1810) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.395 | 2.990 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 3.085 | |
Number of reflections | 53161 | |
<I/σ(I)> | 10.7 | 0.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 26% EDO_P8K (Molecular Dimensions), 0.1M Tris-Bicine pH8.5, 0.1M amino acids mix (Molecular Dimensions), 0.01M Calcium chloride |