5EMS
Crystal Structure of an iodinated insulin analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 233 |
| Detector technology | CCD |
| Collection date | 2010-11-10 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.430, 61.630, 58.580 |
| Unit cell angles | 90.00, 111.38, 90.00 |
Refinement procedure
| Resolution | 40.847 - 2.300 |
| R-factor | 0.1696 |
| Rwork | 0.163 |
| R-free | 0.23260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1znj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.089 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.850 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.054 |
| Number of reflections | 13255 |
| <I/σ(I)> | 18.2 |
| Completeness [%] | 95.6 |
| Redundancy | 4.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M sodium citrate, 0.08% zinc acetate, 2% phenol |
