5EMC
Transcription factor GRDBD and smGRE complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-08 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.980 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.942, 99.643, 112.229 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.256 - 2.300 |
R-factor | 0.2266 |
Rwork | 0.225 |
R-free | 0.26090 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.892 |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.256 | 50.000 | 2.500 |
High resolution limit [Å] | 2.300 | 10.000 | 2.300 |
Rmerge | 0.128 | 0.062 | 0.519 |
Rmeas | 0.154 | 0.076 | 0.630 |
Total number of observations | 57041 | ||
Number of reflections | 19780 | 242 | 4301 |
<I/σ(I)> | 5.45 | 11.44 | 2.05 |
Completeness [%] | 96.6 | 83.7 | 99.1 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 50mM HEPES, 100mM KCl, 10mM MgCl2, 5% PEG 400 |