5EM4
Structure of CYP2B4 F244W in a ligand free conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 31 |
| Unit cell lengths | 92.030, 92.030, 151.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.080 - 3.020 |
| R-factor | 0.231 |
| Rwork | 0.228 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mvr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.647 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.080 | 3.090 |
| High resolution limit [Å] | 3.020 | 3.020 |
| Rmerge | 0.560 | |
| Number of reflections | 24530 | |
| <I/σ(I)> | 2.5 | 1.1 |
| Completeness [%] | 96.7 | 93.3 |
| Redundancy | 6.9 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.1 M HEPES pH 7.0 and 10% w/v polyethylene glycol 6,000 |
