5ELJ
Isoform-specific inhibition of SUMO-dependent protein-protein interactions
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.230, 70.720, 83.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.001 - 1.983 |
R-factor | 0.1906 |
Rwork | 0.188 |
R-free | 0.23630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2UYZ and 4N6T |
RMSD bond length | 0.007 |
RMSD bond angle | 1.012 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.700 | 2.030 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.061 | 0.438 |
Number of reflections | 15526 | |
<I/σ(I)> | 19.3 | 5.2 |
Completeness [%] | 99.4 | 99.1 |
Redundancy | 12.3 | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1 M Hepes sodium salt, 10% w/v polyethylene glycol 20000 |