5EKV
Co-crystal structure of eIF4E with nucleotide mimetic inhibitor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.480, 100.780, 136.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.390 - 3.610 |
| R-factor | 0.2701 |
| Rwork | 0.267 |
| R-free | 0.34170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v8w |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.764 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 81.068 | 50.390 | 3.810 |
| High resolution limit [Å] | 3.610 | 11.420 | 3.610 |
| Rmerge | 0.082 | 0.423 | |
| Rmeas | 0.336 | ||
| Rpim | 0.179 | 0.059 | 0.264 |
| Total number of observations | 21083 | 683 | 2987 |
| Number of reflections | 6413 | ||
| <I/σ(I)> | 4.6 | 10 | 3.1 |
| Completeness [%] | 97.8 | 96.6 | 95.5 |
| Redundancy | 3.3 | 2.8 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 21-31% PEG 8000, 1-3% (NH4)2SO4, 100 mM HEPES, pH 7.5, 1 round of seeding |
