5EK7
Structure of the autoinhibited Epha2 JMS-KD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.475, 75.242, 80.483 |
| Unit cell angles | 90.00, 112.67, 90.00 |
Refinement procedure
| Resolution | 26.806 - 1.901 |
| R-factor | 0.1725 |
| Rwork | 0.171 |
| R-free | 0.20510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.146 |
| Data reduction software | SCALEPACK |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.060 | 0.045 | 0.304 |
| Rmeas | 0.071 | 0.054 | 0.359 |
| Rpim | 0.038 | 0.029 | 0.189 |
| Total number of observations | 198736 | ||
| Number of reflections | 54658 | ||
| <I/σ(I)> | 12.6 | ||
| Completeness [%] | 99.7 | 99.4 | 99.7 |
| Redundancy | 3.6 | 3.5 | 3.5 |
| CC(1/2) | 0.994 | 0.901 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 4% Tacsimate, 5%(w/v) PEG3350, 5% 2-propanol |
