5EK7

Structure of the autoinhibited Epha2 JMS-KD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-27
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	63.475, 75.242, 80.483
Unit cell angles	90.00, 112.67, 90.00

Refinement procedure

Resolution	26.806 - 1.901
R-factor	0.1725
Rwork	0.171
R-free	0.20510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.146
Data reduction software	SCALEPACK
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.970
High resolution limit [Å]	1.900	4.090	1.900
Rmerge	0.060	0.045	0.304
Rmeas	0.071	0.054	0.359
Rpim	0.038	0.029	0.189
Total number of observations	198736
Number of reflections	54658
<I/σ(I)>	12.6
Completeness [%]	99.7	99.4	99.7
Redundancy	3.6	3.5	3.5
CC(1/2)		0.994	0.901

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	4% Tacsimate, 5%(w/v) PEG3350, 5% 2-propanol