5EK0
Human Nav1.7-VSD4-NavAb in complex with GX-936.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 169.420, 188.830, 171.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.590 - 3.530 |
| R-factor | 0.2442 |
| Rwork | 0.243 |
| R-free | 0.27190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rvy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.590 | 3.950 |
| High resolution limit [Å] | 3.530 | 3.530 |
| Rmerge | 0.149 | 0.800 |
| Number of reflections | 33851 | |
| <I/σ(I)> | 10.4 | 2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 9 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 277 | 2.2 ammonium sulfate, 100 mM sodium citrate pH 5.4 |
