5EIK
Structure of a Trimeric Intracellular Cation channel from C. elegans in the absence of Ca2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.53062 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 126.340, 126.340, 135.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.300 |
| Rwork | 0.214 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5egi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.634 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.730 | |
| Number of reflections | 18464 | |
| <I/σ(I)> | 8.6 | 2 |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 5.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 289 | 20% PEG400, 50 mM NaAc buffer (pH 4.4), 50 mM MgAc2, 10 mM betaine hydrochloride |
