5EIG
Engineered human cystathionine gamma lyase (E59T, E339V) to deplet cysteine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97733 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 113.377, 163.455, 181.625 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.700 |
| R-factor | 0.1925 |
| Rwork | 0.191 |
| R-free | 0.23260 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.874 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.545 | |
| Number of reflections | 92940 | |
| <I/σ(I)> | 10.385 | 2.182 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 100mM MES pH6.5 50-100mM Sodium Citratre 25%-30% Isopropanol |
