5EGP
Crystal structure of the S-methyltransferase TmtA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.100, 109.210, 59.940 |
| Unit cell angles | 90.00, 112.44, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.500 |
| R-factor | 0.13397 |
| Rwork | 0.132 |
| R-free | 0.16393 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.349 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.530 |
| Number of reflections | 89458 | |
| <I/σ(I)> | 14 | |
| Completeness [%] | 95.9 | 94.8 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M potassium acetate, 2.2 M ammonium sulfate |
