5EFM
Beclin 1 Flexible-helical Domian (FHD) (141-171)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-06-26 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.3776 |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 63.163, 63.163, 63.163 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.660 - 1.950 |
| R-factor | 0.2119 |
| Rwork | 0.212 |
| R-free | 0.21640 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.298 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.660 | 2.050 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 3490 | |
| <I/σ(I)> | 26.3 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 75.5 | 78.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.6 | 293 | 250mM potassium sodium tartrate tetrahydrate, 2.2 M ammonium sulfate, 100mM sodium citrate tribasic dihydrate (pH 5.6) |
