5EF7
Crystal structure of Danio rerio histone deacetylase 6 catalytic domain 2 in complex with HPOB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 |
Unit cell lengths | 48.672, 56.637, 74.784 |
Unit cell angles | 106.45, 90.11, 97.09 |
Refinement procedure
Resolution | 50.545 - 1.900 |
R-factor | 0.1825 |
Rwork | 0.181 |
R-free | 0.22060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eek |
RMSD bond length | 0.008 |
RMSD bond angle | 1.086 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.600 | 2.050 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.143 | 0.754 |
Number of reflections | 58401 | |
<I/σ(I)> | 6.9 | 1.6 |
Completeness [%] | 97.5 | 95.7 |
Redundancy | 3.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1 M Tris, pH 8.5, 25% PEG3350 |