5EE5
Structure of human ARL1 in complex with the DCB domain of BIG1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 84.166, 50.727, 103.837 |
| Unit cell angles | 90.00, 111.98, 90.00 |
Refinement procedure
| Resolution | 29.069 - 2.279 |
| R-factor | 0.2193 |
| Rwork | 0.217 |
| R-free | 0.25900 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.158 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1810)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.069 | 2.360 |
| High resolution limit [Å] | 2.279 | 2.280 |
| Rmerge | 0.048 | 0.667 |
| Number of reflections | 18726 | |
| <I/σ(I)> | 12.4 | 1.7 |
| Completeness [%] | 97.3 | 94.1 |
| Redundancy | 3.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 8K/PEG 1k 10% Sodium Acetate 0.1-0.2mM Tris pH8.5 glacial acetic 0.100mM |
