5E8M
Crystal structure of human heparanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.765, 70.972, 78.948 |
| Unit cell angles | 90.00, 94.17, 90.00 |
Refinement procedure
| Resolution | 46.640 - 1.750 |
| R-factor | 0.16596 |
| Rwork | 0.164 |
| R-free | 0.19989 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.735 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.640 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.050 | 0.847 |
| Number of reflections | 49329 | |
| <I/σ(I)> | 12 | 1.7 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M MES pH 5.5, 0.1 M MgCl2, 17% PEG3350 |
