5E7Y
Crystal structure of P450 BM3 heme domain M7 variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8123 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.510, 153.890, 61.590 |
| Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.1898 |
| Rwork | 0.188 |
| R-free | 0.21995 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.461 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 74069 |
| <I/σ(I)> | 22 |
| Completeness [%] | 99.7 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100-160 mM MgCl2 100mM MES 6.5 10-18% PEG3350 |
