5E6U
Structures of leukocyte integrin aLb2: The aI domain, the headpiece, and the pocket for the internal ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-18 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.03320 |
Spacegroup name | P 61 |
Unit cell lengths | 154.980, 154.980, 115.450 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.443 - 2.500 |
R-factor | 0.1802 |
Rwork | 0.179 |
R-free | 0.22550 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.762 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 54239 |
<I/σ(I)> | 6.2 |
Completeness [%] | 99.5 |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Mg-formate dehydrate and 15% PEG 3350 |