5E6U

Structures of leukocyte integrin aLb2: The aI domain, the headpiece, and the pocket for the internal ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-18
Detector	MAR CCD 165 mm
Wavelength(s)	1.03320
Spacegroup name	P 61
Unit cell lengths	154.980, 154.980, 115.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.443 - 2.500
R-factor	0.1802
Rwork	0.179
R-free	0.22550
RMSD bond length	0.007
RMSD bond angle	0.762
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	54239
<I/σ(I)>	6.2
Completeness [%]	99.5
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Mg-formate dehydrate and 15% PEG 3350