5E6M
Crystal structure of human wild type GlyRS bound with tRNAGly
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.997 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 206.864, 122.200, 84.727 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.558 - 2.927 |
R-factor | 0.1959 |
Rwork | 0.194 |
R-free | 0.24310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4RK3 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.851 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.060 |
High resolution limit [Å] | 2.927 | 2.927 |
Rmerge | 0.122 | 0.878 |
Number of reflections | 46845 | |
<I/σ(I)> | 11.7 | 1.8 |
Completeness [%] | 96.6 | 96.2 |
Redundancy | 5.2 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 32% PEG 600, 0.1 M NaCl, and 0.1 M MES (pH 6.5) with additives |