5E6H
A Linked Jumonji Domain of the KDM5A Lysine Demethylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-19 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.365, 61.591, 46.583 |
| Unit cell angles | 90.00, 92.55, 90.00 |
Refinement procedure
| Resolution | 32.596 - 2.238 |
| R-factor | 0.1809 |
| Rwork | 0.179 |
| R-free | 0.21990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MANUFACTURED MODEL |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.680 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.600 | 2.320 |
| High resolution limit [Å] | 2.238 | 2.238 |
| Rmerge | 0.241 | 0.603 |
| Number of reflections | 15751 | |
| <I/σ(I)> | 13.3 | 3.7 |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 11.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 1.5 M Ammonium sulfate 100mM Tris-HCl, pH 8.5 12% glycerol |
